Skip navigation
Please use this identifier to cite or link to this item:
Title: Surface Enhanced Raman Spectroscopy of Lactoferrin Adsorbed on Silvered Porous Silicon Covered with Graphene
Authors: Zavatski, S. A.
Khinevich, N. V.
Girel, K. V.
Redko, S. V.
Kovalchuk, N. G.
Komissarov, I. V.
Lukashevich, U. A.
Semak, Igor
Mamatkulov, Kahramon
Vorobyeva, Maria
Arzumanyan, G. M.
Bandarenka, H. V.
Keywords: публикации ученых;surface enhanced Raman scattering;silver;porous silicon;graphene;lactoferrin;attomolar concentration
Issue Date: 2019
Publisher: MDPI
Citation: Surface Enhanced Raman Spectroscopy of Lactoferrin Adsorbed on Silvered Porous Silicon Covered with Graphene / Sergey Zavatski [and other] // Biosensors. – 2019. – Vol. 9. – P. 34 (19 pages). – DOI: 10.3390/bios9010034.]
Abstract: We registered surface enhanced Raman scattering (SERS) spectra of the human lactoferrin molecules adsorbed on a silvered porous silicon (por-Si) from 10^-6–10^-18 M solutions. It was found that the por-Si template causes a negative surface potential of silver particles and their chemical resistivity to oxidation. These properties provided to attract positively charged lactoferrin molecules and prevent their interaction with metallic particles upon 473 nm laser excitation. The SERS spectra of lactoferrin adsorbed from 10^-6 M solution were rather weak but a decrease of the concentration to 10^-10 M led to an enormous growth of the SERS signal. This effect took place as oligomers of lactoferrin were broken down to monomeric units while its concentration was reduced. Oligomers are too large for a uniform overlap with electromagnetic field from silver particles. They cannot provide an intensive SERS signal from the top part of the molecules in contrast to monomers that can be completely covered by the electromagnetic field. The SERS spectra of lactoferrin at the 10^-14 and 10^-16 M concentrations were less intensive and started to change due to increasing contribution from the laser burned molecules. To prevent overheating the analyte molecules on the silvered por-Si were protected with graphene, which allowed the detection of lactoferrin adsorbed from the 10^-18 M solution.
Appears in Collections:Публикации в зарубежных изданиях

Files in This Item:
File Description SizeFormat 
Zavatski_Surface.pdf4.72 MBAdobe PDFView/Open
Show full item record

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.